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Modeling of Photochemical Reactions in a Focused Laser Beam: A Reprint from the “Journal of Research of the National Institute of Standards and Technology”
A.K. Gaigalas (au); F.Y. Hunt (au); L. Wang (au)
Fluorescent materials play a prominent role in the measurement of scientific phenomena. Photodegradation of fluorophores is a process that determines the accuracy and sensitivity of such measurements. Recently, illumination consisting of short pulses has been used to examine the decay of photochemical reaction products. However, the time resolved measurements are difficult to interpret since the photodegradation process usually involves multiple time scales. The frequency domain measurement technique discussed here looks at the frequency response of a fluorescent sample to a frequency modulate illuminating light. In the measurements described here, a focused laser beam is used to illuminate a sample of slowly flowing fluorescent solution. Illus.
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